News | Research

Nanoblades: innovative shuttles for genome editing

On The April 4, 2019

Press release - Ecofect researchers have developed a new delivery system for the CRISPR machinery based on viral pseudoparticles, called Nanoblades.

Emiliano Ricci, Philippe Mangeot and Théophile Ohlmann from the International Center for Research on Infectious Diseases (Lyon), holders of an Ecofect 'Proof of Concept' grant, developed innovative capsules to bring CRISPR / Cas9 to the target DNA. Described in Nature Communications, Nanoblades open up avenues of research for genome editing in human stem cells.

Ecofect researchers based at the International Center for Research on Infectious Diseases (Inserm, CNRS, University Claude Bernard Lyon 1 and ENS Lyon) have developed Nanoblades, particles that can deliver the CRISPR / Cas9 machinery into many cell types, including human cells. They were tested on mice and patented in 2016 by Inserm-Transfert.
The scientists came up with the idea of ​​encapsulating the CRISPR / Cas9 system in structures that are very similar to viruses and thus ensure its delivery within a target cell, by fusion with the cell membrane. To design these Nanoblades, researchers exploited the properties of the retroviral GAG protein, which has the ability to produce non-infectious viral particles because they lack a genome. The research team fused the GAG ​​protein of a mouse retrovirus with the CAS9 protein - the CRISPR system scissors. This new protein called "fusion" is the originality of the Nanoblades.

Press release

Original press release [in french]

Reference article

Genome editing in primary cells and in vivo using viral-derived“Nanoblades” loaded with Cas9/sgRNA ribonucleoproteins
Philippe E. Mangeot, Valérie Risson, Floriane Fusil, Aline Marnef, Emilie Laurent,Juliana Blin, Virginie Mournetas, Emmanuelle Massouridès, Thibault J. M. Sohier, Antoine Corbin, Fabien Aubé, Marie Teixeira, Christian Pinset, Laurent Schaeffer, Gaëlle Legube, François-Loïc Cosset, Els Verhoeyen, Théophile Ohlmann, Emiliano P.Ricci.
Nature Communications 2019, 10:45 -  PDF download